Fig. 1: Sema3a/SEMA3A is expressed in LSECs and increased in mice with hepatic steatosis.
From: Semaphorin-3A regulates liver sinusoidal endothelial cell porosity and promotes hepatic steatosis
a, Sema3a-g mRNA expression (log10 fold change) in LSECs compared to total liver of 14-week-old male C57BL/6 wild-type (wt) mice (n = 8, n = 7 for Sema3e). Lines indicate the same mouse. b, Sema3a-g mRNA expression (log10 fold change, log(0) values are not displayed) in liver tissue of 12-week-old db/db mice, compared to db/+ controls (RT–qPCR; n = 8 each). A multiple two-tailed paired (a) or unpaired (b) Student’s t-test was used to discover significant effects84. Discoveries are indicated by q values in a,b. c, Sema3a mRNA expression in liver tissue from standard chow-fed (n = 4, RT–qPCR) versus HFD-fed (n = 10) littermates. d, Sema3a mRNA expression of LSECs from 12-week-old male db/+ control versus db/db mice (n = 7 each). e, Sema3a mRNA expression of LSECs from 12-week-old male wt control versus ob/ob mice (n = 8 each). f, Relative Sema3a mRNA expression in hepatocytes and LSECs isolated from three and two human donors, respectively (RT–qPCR). g, Graphical overview of SEMA3A and its known receptors. h, Representative immunofluorescent staining for (I and II) neuropilin-1 (red, NRP1), (I and III) LYVE1 (green) and (III) goat IgG isotope control (red) of liver sections of C57BL/6 wt mice (n = 2 mice). Scale bars, 20 µm. i, Agarose gel with PCR products (RT–PCR) showing the expression of several SEMA3A receptors in primary mouse (n = 2 LSEC isolation) and human LSECs (male LSEC donor QC-12B15F11). Brightness and contrast have been adjusted to enhance visibility in h,i. j,k, Nrp1 (j) and Nrp2 (k) mRNA expression in LSECs compared to total liver from 14-week-old male C57BL/6 wt mice (n = 8 each). l,m, Nrp1 and Nrp2 mRNA expression in LSECs from db/db (l; n = 7) and ob/ob mice (m; n = 8) in comparison to controls. A two-tailed unequal variances t-test was used (c–e,l,m) and two-tailed paired t-test (j,k). Data are presented as mean ± s.e.m. CD146+ LSECs were isolated by MACS or FACS after MACS (d,e,l,m) to get an even higher purity of cells.
