Extended Data Fig. 4: Smad6 knockdown in HUVECs.
From: A KLF2-BMPER-Smad1/5 checkpoint regulates high fluid shear stress-mediated artery remodeling
(a–c) HUVECs transfected with control or Smad6 siRNA were subjected to FSS at indicated magnitudes for 12 h. Smad6 knockdown efficiency was confirmed by Q-PCR (a), n = 3 experiments, data are presented as means ± s.e.m. Cells were fixed and stained for Smad1. Nucleus/Cytoplasm intensity ratio of Smad1 was quantified, n = 60 cells for each group from 3 experiments. (d, e). Immunostaining of phospo-Smad1/5. Nucleus intensity of Smad1/5 was quantified (normalized to siCtrl 3 dyn/cm2 group), n = 60 cells for each group from 3 experiments, data showing all points from min to max. Scale bar: 25 μm. Dots represent values from individual cell; center line indicates median (50% percentile); whiskers indicate minimum to maximum; box bounds indicate interquartile range (c, e). Statistics calculated by two-tailed unpaired t tests (a) or two-way ANOVA with Tukey’s multiple comparison tests (c, e). ns, not significant.
