Extended Data Fig. 5: BMPER mediates high FSS-induced Smad1/5 suppression in HAECs.
From: A KLF2-BMPER-Smad1/5 checkpoint regulates high fluid shear stress-mediated artery remodeling
(a–c) Human aortic endothelial cells (HAECs) transfected with control or BMPER siRNA were subjected to FSS at indicated magnitudes for 12 h. BMPER knockdown efficiency was confirmed by western blotting (a). Cells were fixed and stained for Smad1. Nucleus/Cytoplasm intensity ratio of Smad1 was quantified, n = 60 cells for each group from 3 experiments. (d, e). Immunostaining of phospo-Smad1/5. Nucleus intensity of Smad1/5 was quantified (normalized to siCtrl 3 dyn/cm2 group), n = 60 cells for each group from 3 experiments. Scale bar: 25 μm. Dots represent values from individual cell; center line indicates median (50% percentile); whiskers indicate minimum to maximum; box bounds indicate interquartile range (c, e). Statistics calculated by two-way ANOVA with Tukey’s multiple comparison tests (c, e). ns, not significant.
