Extended Data Fig. 10: Immunostaining in T2D mice after BMP9/10 blocking Abs treatment and pathway diagram.
From: A KLF2-BMPER-Smad1/5 checkpoint regulates high fluid shear stress-mediated artery remodeling
(a, b) Representative images and quantification of CD31 staining of calf muscle sections from IgG or BMP9/10 bAbs treated T2D mice. Scale bar: 75 μm. (c, d) Representative images and quantification of p-Akt S473 staining of thigh muscle sections from IgG or BMP9/10 bAbs treated T2D mice. (e, f) Representative images and quantification of BMPER staining of thigh muscle sections from IgG or BMP9/10 bAbs treated T2D mice Scale bar: 75 μm. Right, unligated; Left, ligated. n = 5 mice for both male and female, data are presented as means ± s.e.m. Statistics calculated by two-way ANOVA with Sidak’s multiple comparison tests. ns, not significant. (g) Pathway diagram: high FSS suppresses Smad1/5 activation through KLF2-dependent induction of BMPER, which de-represses Akt to enable vessel remodeling.
