Extended Data Fig. 1: SMC-specific NOTCH1 or MFN1/2 KO validation and ultrasound of aorta in SMC-Notch1−/− and Myh11-CreERT2 mice.
From: Correcting mitochondrial loss mitigates NOTCH1-related aortopathy in mice
(a) Representative images of immunohistochemistry staining with NOTCH1 in the aorta of control and SMC-Notch1−/− mice. (b) Quantitative PCR results of control and SMC-Notch1−/− mice aortas after removing aortic adventitia (n=3). (c) Schematic detailing the locations measured during echocardiographic analysis, and schematic illustrating the calculation of aortic strain and aortic pulse wave velocity. (d) Representative aortic ultrasound images of control mice and SMC-Notch1−/− mice without or with tamoxifen injection (n=5 per group). (e) Representative images of immunohistochemistry staining with MFN1 or MFN2 in the aorta of control and SMC-Mfn1/2−/− mice (n=3 per group). Data are represented as mean ± standard deviation, p values were determined by two-tailed Studentʼs t-tests. * represents P<0.05, ** represents P<0.01. All mice were male.
