Extended Data Fig. 3: TRPM7 is upregulated in human and mouse AAA.
From: The TRPM7 chanzyme in smooth muscle cells drives abdominal aortic aneurysm in mice
(a) Immunofluorescence of TRPM7 in infrarenal aorta (InfraAo) collected from brain-dead organ donors (Ctrl) or AAA patients undergoing open surgical repair. EVG staining was simultaneously performed to indicate the identity of human aorta. n = 3. (b) Typical time course of recorded TRPM7 currents in suprarenal aorta (SupraAo) SMC isolated from saline- or Ang II-infused (14 days) Apoe-/- mice. FTY720 (3 μM) was perfused after the currents level off. The two traces are intentionally aligned by the start point of FTY720 application for back-to-back comparison. (c-d) Typical TRPM7 current traces as indicated. (e) Statistics of TRPM7 current density as indicated. n = 8. (f) SupraAo elastic fiber disruption scoring based on EVG staining in saline or Ang II-infused mice (Related to Fig. 1h). n = 6. (g) SupraAo media SM22α-positive cell proportion (Related to Fig. 1h). n = 6. (h) SupraAo CD45-positive cell proportion (Related to Fig. 1h). n = 6. (i) SupraAo DHE-positive cell count (Related to Fig. 1h). n = 6. (j) InfraAo elastic fiber disruption scoring based on EVG staining in saline or PPE-perfused mice (Related to Fig. 1m). n = 6. (k) InfraAo media SM22α-positive cell proportion (Related to Fig. 1m). n = 6. (l) InfraAo CD45-positive cell proportion (Related to Fig. 1m). n = 6. (m) InfraAo DHE-positive cell count (Related to Fig. 1m). n = 6. The differences were analyzed using two-way ANOVA, followed by Tukey’s post-hoc test (Extended Data Figs. 3e–m).
