Extended Data Fig. 8: SMC-specific Trpm7 deletion suppresses MTF1 and KLF4 signaling.
From: The TRPM7 chanzyme in smooth muscle cells drives abdominal aortic aneurysm in mice
(a) Immunofluorescence of MTF1 in InfraAo from PPE-perfused Ai9sm-tdT/0 mice. MTF1 was shown in green, tdTomato autofluorescence in red and DAPI in blue. MTF1 nuclear localization (white arrows) in SMC was analyzed based on nuclear staining (cyan) in tdTomato positive cell (red). Scale bar=50μm. n = 3. (b) Immunofluorescence of MTF1 in InfraAo from saline or PPE-perfused mice. Analysis is identical to Fig. 5f. n = 3. Scale bar=50μm. (c-d) Detection of MTF1 in extracted cytosolic or nuclear protein from WT or M7KO cells treated with saline or Ang II. n = 3. (e) Immunoblots of MTF1 in extracted cytosolic or nuclear protein from HEK293 cells transfected with empty vector, M7Ch-WT or M7Ch-PD upon naltriben stimulation. (f-g) Statistics of Extended Data Fig. 8e. n = 3. (h) Immunofluorescence of KLF4 in SupraAo from saline or Ang II-infused mice. Scale bar=50μm. n = 3. The differences were determined by two-way ANOVA (Fig. 8c, 8d) or one-way ANOVA (Fig. 8f, 8g), followed by Tukey’s post-hoc test.
