Extended Data Fig. 5: Aging-associated DEG analysis reveals elevated inflammatory and cellular interaction in the aged heart.
From: ARID5A orchestrates cardiac aging and inflammation through MAVS mRNA stabilization
a, Heatmap showing the number of upregulated (purple) and downregulated (green) aging-related DEGs in each cell type. b, Augur was used to prioritize cell types in human cardiac tissue under the influence of age factors. c, The network showing representative GO terms and pathways of aging-related upregulated (up) and downregulated (bottom) DEGs between aged and young human cardiac tissue. The node size indicates the total number of hits for a specific term, and lines connect those with a similarity > 0.3. d, The bar plots display enriched representative Gene Ontology (GO) terms or pathways for aging-related up-regulated (left) and down-regulated (right) differentially expressed genes (DEGs) in cardiomyocytes, fibroblasts, and endothelial cells in human heart tissue. e, Bar plot showing the proportion of CDKN1A- (left), CDKN2A- (middle), and CDKN2B-positive (right) cells in human cardiac tissue across age groups. The data were acquired from 16 young samples and 16 aged samples. Statistical significance was assessed using an unpaired two-tailed t-test. f, The circular heatmap showing the expression fold change (aged vs. young) of inflammation-related gene set for each cell types. The red indicates up-regulation in the aged samples, while green denotes downregulation. The intensity of color reflects the level of overexpression in aged cells for each gene. g, The circular heatmap showing the expression fold change (aged vs. young) of senescence-associated secretory phenotype (SASP)-related genes for each cell types. The red indicates up-regulation in the aged samples, while green denotes downregulation. The intensity of color reflects the level of overexpression in aged cells for each gene. h, Immunohistochemical staining of S100A9 in young- and aged- human right cardiac tissues. Scale bars, 20 μm and 5 μm (zoomed-in image). Data are presented as mean ± s.e.m., n = 10, **P < 0.01. Statistical analysis was conducted by using unpaired student’s t-test (two-tailed).
