Fig. 5: Comparing the transcriptomic profiling of the right ventricle and septum in the confirmatory cohort of patients with CTEPH before and after PEA. | Nature Cardiovascular Research

Fig. 5: Comparing the transcriptomic profiling of the right ventricle and septum in the confirmatory cohort of patients with CTEPH before and after PEA.

From: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery

Fig. 5: Comparing the transcriptomic profiling of the right ventricle and septum in the confirmatory cohort of patients with CTEPH before and after PEA.

a, In the confirmatory cohort, gene expression profiles of paired B-prePEA_RV and B-postPEA_septum samples were compared. RNA-seq analysis was performed on 21 RV and septum tissues of patients with CTEPH. Patients at prePEA were categorized into moderate-risk (B-prePEAm_RV, n = 9), intermediate-risk (B-prePEAi_RV, n = 8) and severe-risk (B-prePEAs_RV, n = 4) groups based on their clinical parameters and ESC/ERS guidelines. After 12 months, postPEA_septum biopsies were obtained from the septum tissues of the same patients, including B-postPEAm_septum (n = 9), B-postPEAi_septum (n = 8) and B-postPEAs_septum (n = 4). b, PCA plots depicting the RV and septum transcriptomic profiles of patient-paired samples in the following groups: B-prePEA_RV (n = 21) and B-postPEA_septum (n = 21), B-prePEAm_RV (n = 9) and B-postPEAm_septum (n = 9), B-prePEAi_RV (n = 8) and B-postPEAi_septum (n = 8), and B-prePEAs_RV (n = 4) and B-postPEAs_septum (n = 4). The encircled red dots represent the distinct status of the right ventricle, while the corresponding septum is shown using blue dots. c–f, Volcano plots showing the distribution of each gene in B-postPEA_septum versus B-prePEA_RV (c), B-postPEAm_septum versus B-prePEAm_RV (d), B-postPEAi_septum versus B-prePEAi_RV (e) and B-postPEAs_septum versus B-prePEAs_RV (f). The significant DEGs (base mean expression ≥ 5; |log2(FC)| ≥ 0.585; Padj ≤ 0.05, two sided) were classified based on P < 0.05 and |log2(FC)| > 0.585 (red dots), only P value (blue dots), log2(FC) (green dots) and not significant (gray dots). g, Top: Venn diagram illustrating the shared and distinct DEGs in four sets as follows: B-postPEA_septum versus B-prePEA_RV, B-postPEAm_septum versus B-prePEAm_RV, B-postPEAi_septum versus B-prePEAi_RV, and B-postPEAs_septum versus B-prePEAs_RV. Bottom: the common and distinct DEGs among three groups—B-postPEAm_septum and B-prePEAm_RV, B-postPEAi_septum and B-prePEAi_RV, and B-postPEAs_septum and B-prePEAs_RV—are highlighted. h, Heatmap illustrating the top 20 overrepresented clusters of terms for all the DEGs between several comparisons: B-postPEAi_septum versus B-prePEAi_RV, B-postPEAm_septum versus B-prePEAm_RV, B-postPEA_septum versus B-prePEA_RV, and B-postPEAs_septum versus B-prePEAs_RV.

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