Fig. 7: Inhibition of SERPINE1, IL7R and ANKRD1 in HCFs and HCMECs.

a, HCMECs were transfected with siRNA for 48 h, followed by a 24 h tube formation assay. The representative images were taken at 1 h and 24 h. The number of junctions and total tube length were analyzed and quantified using ImageJ software. Data are presented as mean ± SEM. (n = 3 biological replicates; Kruskal–Wallis test, **P = 0.0021 compared with scrambled siRNA). Scale bars, 1 mm. The boxes show the IQR (25th to 75th percentile), and the central bands indicate the median. The whiskers extend to 1.5 times the IQR above and below the box. b, HCFs were transfected with siRNA for 48 h, followed by a 24 h tube migration assay. Representative images were taken at 1 h and 24 h, and the percentage of wound closure was analyzed and quantified using ImageJ software with the wound healing analyzer plugin. A scrambled siRNA pool was used as a negative control. The data are presented as mean ± SEM. (n = 3 biological replicates with only one having one technical replicate; two-tailed unpaired t-test). Scale bars, 1 mm. The boxes show the IQR (25th to 75th percentile), and the central bands indicate the median. The whiskers extend to 1.5 times the IQR above and below the box. c, HCFs were transfected with siRNA for 48 h, and the proliferation was measured using BrdU incorporation assay. Data are presented as mean ± SEM. (n = 4 biological replicates and 6 technical replicates; two-tailed unpaired t-test). Black empty circles represent cells transfected with siCONTROL, and red empty circles represent cells transfected with target siRNA.