Fig. 4: MRI of vascular disruption in rabbit stroke model confirms protective effects of SKI-1 inhibitor PF-429242.
From: Inhibition of proprotein convertase SKI-1 prevents blood vessel alteration after stroke
a, Angiograph of contrast agent (iodixanol) injection before and after embolization. b,c, MRI of vehicle-treated (n = 5) and PF-429242-treated (n = 6) rabbits 2 days after infarction. b, DWI of infarcted regions. Quantification: infarct volume computed from segmentation of b1000 hyperintense regions. c, Patlak modeling of vascular permeability (Ktrans) and plasma volume (Vp) from DCE imaging of gadolinium contrast agent accumulation. Quantification: relative difference in Ktrans values between the ipsilateral and contralateral hemispheres. d, TTC-stained coronal brain slices showing the representative infarcted area in vehicle-treated (n = 5) and PF-429242-treated (n = 6) rabbits. Infarcted metabolically inactive tissue appears white and was quantified using Yang’s normalization scheme. e, NSS evaluated until 7 days after embolization. All graphs indicate mean ± s.e.m. Significance of b–d was assessed with two-tailed unpaired t-tests. Each dot represents an independent experiment. Time series e was assessed with repeated-measures factorial ANOVA (between-group P value in the legend), and two-tailed unpaired t-tests were applied to individual timepoints. Each dot represents an independent experiment for a given timepoint. CT, computed tomography; Gd, gadolinium.
