Fig. 8: Disruption of RGMa–Neo1 signaling pathway reduced behavioral deficits observed over 7 days after MCAO.
From: Inhibition of proprotein convertase SKI-1 prevents blood vessel alteration after stroke
a,b, Open field assessment for total activity and distance traveled, respectively. Amonlite system diagram is shown in Extended Data Fig. 6b. c, NSS. d, Forelimb placing score both contralateral forelimb (controlled by infarcted ipsilateral cortex) and ipsilateral forelimb (controlled by contralateral cortex). e,f, Neurological deficits and seizure activity after stroke assessed with Bederson and Racine scoring systems, respectively. Neurological scores c,e improved in RGMaF155A/+ and NeoΔTie2-creERT2+TAM groups 3–7 days after MCAO, relative to their respective controls. Racine scores f improved at 72 hours after MCAO. For all graphs, time indicates hours or days after MCAO, where t = 0 is prior to MCAO. All graphs indicate mean ± s.e.m. Time series a–e were assessed with repeated-measures factorial ANOVAs (between-group P values in the legends), and two-tailed unpaired t-tests were applied to individual timepoints. Each dot represents an independent experiment at a given timepoint. Time series f was assessed with a two-tailed unequal-variance t-test applied to cumulative subject scores (P values in the legends) and similarly for individual timepoint scores. Each dot represents a subject with non-zero score on a given day.
