Extended Data Fig. 4: KLF4 expression negatively correlates with cushion EndoMT progression.
From: Endocardial primary cilia and blood flow regulate EndoMT during endocardial cushion development
a) HCR-FISH on whole mount mouse embryos at e9.5 showing Kdr mRNA (red), Vim mRNA (green), and nuclei (Hoechst, blue). The AVC endocardium is outlined in white. b) Correlation matrix between Klf4 and EndoMT regulators in the endocardium; mRNA expression from SingleNuc RNAseq (4,291 nuclei). Abbreviations: OFT-Outflow Tract, WT-Wildtype.
