Extended Data Fig. 8: Defects in blood flow block EndoMT.
From: Endocardial primary cilia and blood flow regulate EndoMT during endocardial cushion development
a) Number of features and counts across biological replicates for the Ncx1−/− snRNAseq dataset (36,296 nuclei). b) Integration of biological replicates for snRNAseq of the whole embryonic e9.5 heart (36,296 nuclei). c) UMAP plot of e9.5 whole hearts (36,296 nuclei). d) Graph of the percentage of cell types in the whole heart (Ncx1+/+ 12,763 nuclei, Ncx1+/− 12,030 nuclei, Ncx1−/− 11,503 nuclei). e) UMAP plot of the endocardial subcluster (6,548 nuclei). f) Graph of the percentage of cell types in the endocardial subcluster (Ncx1+/+ 2,589 nuclei, Ncx1+/− 2,265 nuclei, Ncx1−/− 1,694 nuclei). g) GeneSet Enrichment for EndoMT progression for vEC/EndoMT clusters (2,601 nuclei). h) UMAP plot of the vEC/EndoMT subcluster (2,601 nuclei). i) The percentage of nuclei in each EndoMT pseudostage per genotype (Ncx1+/+ 1,284 nuclei, Ncx1+/− 918 nuclei, Ncx1−/− 399 nuclei). j) Percentage of cells in each cell cycle phase per genotype in the snRNA-seq vEC/EndoMT cluster (2,601 nuclei). k) Violin plots for cell death gene expression in each genotype in vEC/EndoMT cluster (2,601 nuclei). Abbreviations: EC-Endocardium, Endo-EndoMT, HE-Hematoendothelium, Mes-Mesenchyme, MP-Multipotent, PIM-Pro-inflammatory, vEC-Valvular Endocardial Cell, WT-Wildtype.
