Extended Data Fig. 3: Loss of ADAR1 regulates transcriptomic and inflammatory response to calcification in vitro. | Nature Cardiovascular Research

Extended Data Fig. 3: Loss of ADAR1 regulates transcriptomic and inflammatory response to calcification in vitro.

From: Smooth muscle expression of RNA editing enzyme ADAR1 controls activation of the RNA sensor MDA5 in atherosclerosis

Extended Data Fig. 3

Images of hTert immortalized human coronary artery SMCs following treatment with scramble, ADAR, ADAR + IFIH1, and IFIH1 siRNA and 7 days of culture in control medium (a) and calcification medium (b). (c) Volcano plot of DE gene analysis between scramble siRNA treated cells under control and calcification medium. (d-g) Grouped bar charts of normalized RNAseq reads for (d) FBLN5, (e) FMOD, (f) RANBP3L, (g) ISG15. (h) Principal component analysis (PCA) for PC1 and PC2 of bulk RNAseq data from each cell treatment. Grouped bar charts of normalized RNAseq reads for (i) NFKB1, (j) JUND, (k) MMP1, (l) MMP10. P-values represent two-way ANOVA with multiple comparisons post hoc analysis. N = 3 RNA seq libraries per condition.

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