Extended Data Fig. 9: SMC specific haploinsufficiency of Adar1 has minimal effect on macrophage infiltration in atherosclerosis. | Nature Cardiovascular Research

Extended Data Fig. 9: SMC specific haploinsufficiency of Adar1 has minimal effect on macrophage infiltration in atherosclerosis.

From: Smooth muscle expression of RNA editing enzyme ADAR1 controls activation of the RNA sensor MDA5 in atherosclerosis

Extended Data Fig. 9

(a) UMAP of scRNAseq data of non-tdTomato lineage traced cells from atherosclerotic aortic root and ascending aorta at 16 weeks high fat diet split by control and SMC-Adar1−/+ genotypes. (b) Stacked bar chart comparing proportion of macrophage cell population between control and SMC-Adar1−/+ genotypes in the non-tdT+ cells. (c) Heatmap of network centrality scores for CCL signaling network across cell clusters. (d) Dot plot of outgoing and incoming interaction strength across cell clusters for all signaling networks (left) and CCL signaling network (right). (e-f) Cd68 immunohistochemical staining within the aortic root following in control (e) and SMC- SMC-Adar1−/+ (f) mice (N = 13 per group). (g) Total area of Cd68 positive stain showing a significant increase in Cd68 in SMC-Adar1−/+ compared to control, however this effect is not significant when normalizing to (h) plaque area, or (i) vessel area at the internal elastic lamina (IEL). P-values represent chi-squared test (b) and unpaired two-tailed T-test (g-i).

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