Fig. 6: Metabolic changes during ischemia and reperfusion.
From: Rapid mitochondrial repolarization upon reperfusion after cardiac ischemia
Isolated perfused C57BL/6N or Mb−/− mouse hearts (n = 6) were subjected to global ischemia–reperfusion. Whole hearts were rapidly frozen at control, after 20 min of ischemia or the indicated times after reperfusion. a, Succinate was extracted from frozen tissue samples and measured by LC–MS/MS against a [13C4]succinate internal standard. b, CoQH2 and CoQ were extracted from frozen tissue, and the percent reduction was determined by LC–MS/MS by determining the proportion of CoQH2 relative to total CoQ and CoQH2. c, Succinate and CoQH2 data from a and b were fit to interpolated curves and are plotted over time along with cytochrome c absorbance (Fig. 4c) and ΔΨm (Fig. 4i). d, Curves in c replotted to highlight reperfusion. The y axes of c and d represent the range of values observed in the heart in this study. Data presented in a and b are the mean ± s.d.
