Extended Data Fig. 5: scRNA-seq of Tbx5 mutants by region.
From: A disrupted compartment boundary underlies abnormal cardiac patterning and congenital heart defects
a–c, In scRNA-seq samples from E13.5 hearts, we display cells by (a) region (left ventricle (LV), right ventricle (RV), interventricular septu (IVS)) (b) genotype, or (c) cell types by Louvain clustering. In IVS+atrioventricular complex (AVCo) regions (control, n = 4, Tbx5 mutant, n = 2), we detected clusters enriched for (d) Tnnt2+ cardiomyocytes (CMs), (e) Tbx18+/Wt1+ epicardial cells, (f) Plvap+ endothelial cells, (g) Postn+ fibroblasts, (h) Hbb-b2+ red blood cells, and (i) C1qb+ white blood cells. Dot plots of selected differentially expressed genes in (j) tdTomato+ or (k) ZsGreen+ cells from the IVS+AVCo region. Significance of adj p < 0.05 was determined by two-sided Wilcoxan rank sum.
