Extended Data Fig. 2: Mef2cAHF+ lineage distribution in intersectional-DTA mutants.
From: A disrupted compartment boundary underlies abnormal cardiac patterning and congenital heart defects
a, Mef2cAHF+ lineage was enriched in the RV of control embryos (Tbx5CreERT2/+;Mef2cAHF-DreERT2;Hip11+/+) (n = 3) and (b) intersectional-DTA mutant embryos (Tbx5CreERT2/+;Mef2cAHF-DreERT2;Hip11Intersectional-DTA176/+) (n = 2). c, This distribution of the Mef2cAHF+ lineage was not different in the RV or LV between control and DTA mutants (p = 0.939327, by two-sided T-test). Cells of the right ventricle (RV) or left ventricle (LV) in five optical slices at different anterior-posterior planes per embryonic heart sample of 3 controls and 2 mutants were evaluated. Mean and standard deviation are shown. d, Linear profiles showed a reduction of fluorescence activity in the LV of DTA mutants. Linear profiles at 5 apical-basal planes per optical slice, for 5 optical slices at different anterior-posterior planes per embryonic heart sample of 3 controls and 2 DTA mutants were assessed. Statistical significance (p < 0.05) between linear profiles of control and DTA mutants was determined by Welch’s two-sided t-test at each position along the right-left axis. Mean and standard error of the mean are shown. Precise p-values can be found in the Source data. e, f, Cartoon depictions.
