Fig. 5: Drug test using fluorescence imaging on HYDRA hydrogels cast on inexpensive traditional tissue culture plastic. | Communications Engineering

Fig. 5: Drug test using fluorescence imaging on HYDRA hydrogels cast on inexpensive traditional tissue culture plastic.

From: Fabrication of cell culture hydrogels by robotic liquid handling automation for high-throughput drug testing

Fig. 5: Drug test using fluorescence imaging on HYDRA hydrogels cast on inexpensive traditional tissue culture plastic.The alternative text for this image may have been generated using AI.

This is a worst-case configuration for imaging because photons must travel through a thick polystyrene layer in addition to the thin hydrogel layer (see Fig. 6 for a best-case scenario). a Schematic illustration of cell-substrate interaction during the cell cycle. A cell in i) G1 or in ii) S/G2 phase exhibits a degree of spreading based on the substrate. During iii) the mitosis (M) phase, the phase before cell division, the cell undergoes rapid internal architectural changes, assuming the characteristic spherical shape associated with mitotic cells. b Static widefield images of HaCaT (RFP – LifeAct™ in gray, GFP – tagged tubulin in green) 48 hours after seeding on gels cast on tissue culture plastic. Cells were treated with 50 ng mL-1 nocodazole. Arrows stand for (i) cells in the G1 phase (in cyan), (ii) cells in the S/G2 phase (in magenta), and (iii) cells in the mitotic (M) phase. Asterisks stand for (iv) nuclear fragmentation. (v) Fold change in M phase cell number after 48 hours vs. nocodazole concentration on plastic and hydrogel substrates. Cell number in the M phase was counted as a fraction of the total cell number in a FOV and then normalized to the vehicle negative control (n = 12). Data are displayed as mean ± s.e.m. (*) stands for statistical difference. Images were taken on hydrogel thin layers cast on plastic plates. Scale bar: 25 μm.

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