Fig. 3: ∅CAO accurately estimates aberrations in real samples and restores resolution, even in highly distorted images.
From: Phase-based computational adaptive optics enables artifact-free super-resolution microscopy
a Side views of yellow-green 100-nm fluorescent bead images acquired under varying correction collar positions of the objective lens, along with the same images corrected using ∅CAO for spherical aberration (SA). b Plot of the measured SA values from the bead images in a as a function of correction collar scale. The inset shows a top view of the bead image acquired at a collar setting of 185 µm. c Box plots of axial full width at half maximum (FWHM) of the bead images in a, plotted against correction collar scale (n ≥ 10). d Highly aberrated beads images observed through plant tissue were corrected using ∅CAO using 96 Zernike modes and subregional aberration measurement. Maximum intensity projections are shown in top and side views. Subregions used for aberration measurement are indicated by white boxes in the original image.
