Fig. 4: ∅CAO enhances resolution and signal-to-noise ratio (SNR) in wide-field microscopy of live C. elegans embryos.
From: Phase-based computational adaptive optics enables artifact-free super-resolution microscopy
Histone H2B-GFP was expressed in live C. elegans embryos to visualize nuclear chromatin. a Boxed regions indicate areas where optical aberrations were measured. Images are shown as maximum intensity projections along the Z- and Y-axes. b Representative single optical sections of the original and corrected images following Seidel aberration correction. Axial positions at the top denote the distance from the bottom of the animal. c Representative optical sections of the original and ∅CAO-corrected images after deconvolution using three methods: “RL(SHB)”, Richardson-Lucy with Scaled Heavy Ball algorithm47; “eGold”, enhanced Gold algorithm48; “RL”, conventional Richardson-Lucy algorithm. d Line profiles of original and ∅CAO-corrected images derived from the dotted line shown in the side view of the original image deconvolved with “RL(SHB)”. Scale bars: 10 µm for the full-field view in a–c; 2.5 µm for the inset in b, c showing the aberration measurement regions.
