Fig. 2: Regrowth delay and killing of non-growing UPEC.
From: Antibacterial compounds against non-growing and intracellular bacteria
Heatmap showing the regrowth delay and killing of non-growing UPEC CFT073 by validated hit compounds. Gray areas indicate compounds not tested for killing due to weak activity in regrowth-delay experiments. Bacteria were grown in 1:4 CA-MHB (pH 7.4) or LPM (pH 5.5) for 24 h and then treated with the compounds at the indicated concentrations for 24 h. Regrowth data were obtained by measuring OD600 6 h after a 2500-fold dilution of the samples into CA-MHB. Killing data were determined by spot-plating serial dilutions of the treated samples on LB agar plates followed by colony counting. The differences in CFU/mL between the drug-treated samples and the drug-free control are shown. Data represent the mean of three biological replicates. The significance of the regrowth delay was assessed using one-way ANOVA followed by Dunnett’s test, comparing each treatment to the drug-free control. Asterisks denote p-values from the Dunnett’s test.
