Fig. 4: Regrowth delay and killing of non-growing P. aeruginosa and S. aureus.
From: Antibacterial compounds against non-growing and intracellular bacteria
Regrowth delay and killing of non-growing P. aeruginosa DSM1117 and S. aureus DSM2569 by compounds effective against non-growing UPEC. Gray areas in the heat map indicate compounds not tested due to inactivity in preliminary experiments. Cultures were grown in 1:4 CA-MHB (pH 7.4) for 24 h followed by treatment with the compounds at the indicated concentrations for an additional 24 h. To assess regrowth, OD600 was measured on a microplate reader after a 2500-fold dilution into CA-MHB. The time required for cultures to reach an OD600 threshold of 0.12 is reported as the start-growth-time (SGT). Bacterial killing was determined by spot-plating serial dilutions of the treated samples on LB agar plates followed by colony counting. The differences in CFU/mL between drug-treated samples and the drug-free control are presented. Data represent the average of three biological replicates. The significance of regrowth delay was evaluated using OD600 measurements taken 8 h after dilution into drug-free media. Statistical significance was assessed using one-way ANOVA followed by Dunnett’s test, comparing each treatment to the drug-free control. Asterisks denote p-values from Dunnett’s test.
