Fig. 5: Activity of hit compounds against growing and nongrowing bacteria.
From: Antibacterial compounds against non-growing and intracellular bacteria
The activity against growing bacteria was assessed by determining the minimal inhibitory concentration (MIC) using the broth dilution method. The activity against non-growing bacteria was evaluated by calculating the EC50 based on regrowth delay, measured by OD600 readings at 6 h (A, B) or 8 h (C, D) after dilution of treated bacteria into drug-free growth media. EVO evofosfamide, DEG degrasyn, MMC mitomycin C, SOL solithromycin, TYL tylosin, ROS rosamicin, VAL valnemulin. A, C, D 1:4 CA-MHB (pH 7.4) was used for cultivation and treatment of non-growing UPEC CFT073, P. aeruginosa DSM1117, and S. aureus DSM2569. 1:4 CA-MHB (pH 7.4) and CA-MHB (pH 7.4) were used for MIC measurement (Dataset 1). B LPM (pH 5.5) was used in experiments with non-growing E. coli CFT073 and MIC determination (Dataset 1).
