Table 3 Primers used for qPCR in this study
Gene | Primer | Sequence | Amplicon (b.p.) | Tm (°C) |
|---|---|---|---|---|
Housekeeping | ||||
 16S | F R | 5’-ATACCGCATAACGTCGCAAGACCA-3’ 5’-AGCCGTTACCTCACCAAGCTA-3’ | 94 | 64.7 61.2 |
Replication | ||||
 repA | F R | 5’-GCAGAGCTGGGACTGATTAC-3’ 5’-CATCAAGGGCGGCAAATAAC-3’ | 106 | 58.1 57.8 |
 repB | F R | 5’-AGCTGACACGTCTTTCCCTG-3’ 5’-GGCGGGCAAAGGAATGAATG-3’ | 123 | 60.0 60.2 |
Transfer | ||||
 traA | F R | 5’-GGCGGGCAAAGGAATGAATG-3’ 5’-TGAAGTTCTGGTCGGTGCAG-3’ | 101 | 60.2 60.2 |
 traB | F R | 5’-AGCCGAGGATTTTGCCGTTA-3’ 5’-ATCAGTTGCCTGAAGGACGG-3’ | 118 | 60.0 60.0 |
 traC | F R | 5’-CAGCTGGTTACGGTAGGTGG-3’ 5’-TGACCTGCCTGCCCTTTATG-3’ | 167 | 60.1 60.0 |
 traD | F R | 5’-ACCACCATATCACCACGCTG-3’ 5’-TGGCGATCTGCCGATTATCC-3’ | 129 | 60.1 60.0 |
 traE | F R | 5’-AAACCAGAGTGCCCCAACAA-3’ 5’-GCCTCGGAGATCTGTGCTTT-3’ | 75 | 60.0 60.1 |
 traF | F R | 5’-GCCTCGGAGATCTGTGCTTT-3’ 5’-GCAGGCTGGCAGTGGTATAA-3’ | 159 | 60.2 60.1 |
 traG | F R | 5’-TAACTGTCGCCAATCAGCGT-3’ 5’-ATGCCCAGCAGCTATTTGGT-3’ | 70 | 60.0 60.0 |
 traH | F R | 5’-CTGCGGGTCAACCAGGTATT-3’ 5’-ACACGCCTCTGGATGACAAG-3’ | 80 | 60.0 60.0 |
 traI | F R | 5’-AACCCCGTTTTCCCCACTTT-3’ 5’-CTGGCTTCCACACGGGTTAT-3’ | 197 | 60.0 60.0 |
 traJ | F R | 5’-GTGCGCTGGATCGTAGAGAA-3’ 5’-ACAAACCGGAACGGGAGAAT-3’ | 101 | 59.9 59.6 |
 traK | F R | 5’-ATCTGCCACTGAAGACAGCC-3’ 5’-AACGTCCACGCCTTACGAAT-3’ | 139 | 60.0 60.0 |
 traL | F R | 5’-CCAGAACCGCTGCACCAATA-3’ 5’-AACACTGACCAACCAGAGCC-3’ | 125 | 60.7 60.2 |
 traM | F R | 5’-GCGTCATATACACGGAGCCC-3’ 5’-TGAAAAGCGTCGTCAGGAGG-3’ | 96 | 60.7 60.3 |
 traN | F R | 5’-TCCGGATTCGCGTTATAGCC-3’ 5’-AGATCAAAGGGCAGGGAAGC-3’ | 79 | 60.0 60.0 |
 traP | F R | 5’-TTCATCTGCGGTTACCACCC-3’ 5’-GCATTATGGGCAACACTGGC-3’ | 111 | 60.0 60.2 |
 traQ | F R | 5’-CATGCATCCAGCACCTGGTA-3’ 5’-CATATCGTCGCCCGTCTTGT-3’ | 110 | 60.1 60.2 |
 traR | F R | 5’-ACAACGTCACACCGGGAAAT-3’ 5’-AGTGATGAAGCCGATGAAGCA-3’ | 169 | 60.2 60.1 |
 traS | F R | 5’-TCTGCCAGCAAACGAAGAAATG-3’ 5’-CAGGTCATAAAGCCAGGGGG-3’ | 74 | 60.0 60.1 |
 traT | F R | 5’-TTGCGATTGATTTGGCCAGC-3’ 5’-CAACGGATAATGTTGCCGCC-3’ | 180 | 60.1 60.2 |
 traU | F R | 5’-CTGCAACGGACTGGACTCAT-3’ 5’-GCCAGCGCCTTTAATATGCC-3’ | 108 | 60.0 60.0 |
 traV | F R | 5’-TGCTCACCTGTTCTTAGCGG-3’ 5’-CAGAAACTGCTGGCACCTCT-3’ | 116 | 60.0 60.3 |
 traW | F R | 5’-ATTTTCTGCCCTCCTCTGCC-3’ 5’-CAGAAGTCTCTGGACAGCCG-3’ | 151 | 60.0 60.1 |
 traX | F R | 5’-CCGACCAGAAACATCCACGA-3’ 5’-CATCAAAACGGTGGCACTGG-3’ | 87 | 60.0 60.0 |
 traY | F R | 5’-TTCGGCCACCTCCCTGAATA-3’ 5’-GGCGGTATAAGCAGAACGGT-3’ | 177 | 60.6 60.2 |
