Table 1 a. API 20 NE Biochemical Profiles of Streptococcus mutans Lineages. b. API Strep Biochemical Profiles of Streptococcus mutans Lineages

From: Adaptive transcriptional remodeling of Streptococcus mutans under simulated microgravity and silver stress reveals evolutionary innovation in artificial environments

    

Normal Gravity (NG)

Simulated Microgravity (sMG)

Simulated Microgravity and Silver Nitrate (sMGAg)

Test

Active Ingredients

Reactions/Enzymes

Ancestral

1

2

3

4

1

2

3

4

5

6

7

8

1

2

3

4

NO3

potassium nitrate

reduction of nitrates to nitrites

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

 

reduction of nitrates to nitrogen

-

-

-

-

-

-

-

-

-

-

-

-

-

+++

+++

+++

+++

TRP

L-Tryptophane

indole production

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

GLU

D-glucose

fermentation

+++

+++

+++

+++

+++

-

-

-

-

-

-

-

-

-

-

-

-

ADH

L-arginine

arginine dihydrolase

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

URE

urea

urease

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

ESC

esculin ferric citrate

hydrolysis

+++

+++

+++

+++

+++

-

-

-

-

+++

+++

+++

+++

+

+

+

+

GEL

gelatin

hydolysis

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

PNPG

4-nitrophenyl-BD-galactopyranoside

B-galactosidase

+++

+++

+++

+++

+++

-

-

-

-

+++

+++

+++

+++

+++

+++

+++

-

   

Normal Gravity (NG)

Simulated Microgravity (sMG)

Simulated Microgravity and Silver Nitrate (sMGAg)

Active Ingredients

Reactions/Enzymes

Ancestral

1

2

3

4

1

2

3

4

5

6

7

8

1

2

3

4

VP

 

+++

+++

+++

+++

+++

-

-

-

-

-

-

-

-

-

-

-

-

Ribose

fermentation

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

Arabinose

fermentation

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

Manose

fermentation

+++

+++

+++

+++

+++

-

-

-

-

-

-

-

-

-

-

-

-

Sorbitol

fermentation

+++

+++

+++

+++

+++

-

-

-

-

-

-

-

-

-

-

-

-

Lactose

fermentation

+++

+++

+++

+++

+++

-

-

-

-

-

-

-

-

+

-

-

-

Trehalose

fermentation

+++

+++

+++

+++

+++

+++

+++

+++

+++

+++

+++

+++

+++

+++

+++

+++

+++

INU

fermentation

+++

+++

+++

+++

+++

-

-

-

-

-

-

-

-

-

-

-

-

Rafinose

fermentation

+++

+++

+++

+++

+++

-

-

-

-

-

-

-

-

-

-

-

-

AMD

fermentation

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

GLYG

fermentation

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

  1. Positive results for the ancestral strain were denoted as +++, while ++ and + indicate positive reactions of decreasing intensity relative to the ancestral phenotype; – denotes a negative result.
  2. Blue rows indicate phenotypes that differ from the ancestral strain.