Fig. 1: Intranasal inoculation of hDPP4 mice, but not wildtype mice, with MERS-CoV results in disease and viral shedding.
From: Transmission dynamics of MERS-CoV in a transgenic human DPP4 mouse model
Detection of hDPP4 expression in hDPP4 mice using immunohistochemistry in (a) nasal mucosa; (b) trachea; and (c) type I and II pneumocytes, bronchiolar and endothelial cells in lung tissue. d Comparison of hDPP4 expression in lung and kidney tissue obtained from wildtype and hDPP4 mice using flow cytometry. N = 3, bars represent median. e Survival curves of mice after inoculation with 5 × 105 TCID50 MERS-CoV. N = 4 (Wildtype) or 5 (hDPP4). ** = p < 0.01. f Relative weight loss in mice after MERS-CoV inoculation. The lines represent median±range. Mice were euthanized upon reaching >20% of body weight loss (dotted line). g Viral load (gRNA) in oropharyngeal swabs obtained from mice after inoculation with 5 × 105 TCID50 MERS-CoV. h Infectious MERS-CoV titers in lung, brain, and kidney tissue of hDPP4 mice. Dotted line detection limit.
