Fig. 4: Temperature and relative humidity affect VSV-GFP transmission via droplets.
From: Survival and transmission fitness of SARS-CoV-2 over the time-of-flight in an aerosolization chamber
106 IUs of VSV-GFP was aerosolized at various temperatures (4 °C, 25 °C and 36 °C) and relative humidity (RH) (30, 60, and 90%). Droplets were collected on rice paper from the top and bottom trays. Under 18–25 °C and 40% < RH < 60%, the estimated volume collected was 7.9 × 10-4 μL for the top tray and 3.8 × 10-4 μL for the bottom tray. a–f Quantification of infectious virus recovered from rice paper when VSV-GFP was aerosolized at various temperatures with 30, 60, and 90% RH (n = 5). Statistical significance was determined using the Kruskal-Wallis test. The dotted line indicates the lower limit of detection (6.61 TCID50/mL). Each data point was from one aerosolization. Geometric mean is shown.
