Fig. 2: Theoretical resolution improvement with increasing expansion factors.
From: Visualising viral interactions and mechanisms at the nanoscale with expansion microscopy
Theoretical resolution limit calculated for a confocal microscope setup equipped with a 1.2 NA water objective lens, with NA = numerical aperture. The Rayleigh resolution limit is defined as 0.61*λ/NA, with λ = emission wavelength. The resolution is dependent on the emission wavelength and, in first approximation, on the expansion factor.
