Fig. 3: Microscope modalities for imaging expanded samples.
From: Visualising viral interactions and mechanisms at the nanoscale with expansion microscopy
Trade-off among different microscope techniques for imaging physically expanded, biological samples and schematic illustration of their beam path geometries. Different imaging techniques offer distinct advantages but also have some limitations (a). Shown are the optical configurations of (b) widefield microscopy, c confocal laser scanning microscopy, d light sheet fluorescence microscopy, and e oblique plane microscopy. For each technique, the excitation (blue) and detection paths (yellow) are indicated, highlighting differences in excitation geometry, optical sectioning and image generation.
