Fig. 8: Evolution of Chick CAM cannulation methodology.
From: The chicken chorioallantoic membrane as a low-cost, high-throughput model for cancer imaging
Various methods were trialed during cannulation optimization: a Direct injection with 30 g insulin syringe by hand. b Cut 30 g needle with needle holders. c Micromanipulators fixed with a hooked spatula attachment used to pull the vessel over and provide tension. d Glass needle and peristaltic pump tubing tied in with suture. e Glass needles injected by hand at branch points and secured in place using vetbond glue (optimized technique). f Photo of the final glass needle cannula used for all subsequent experiments. g Successful cannulation of a CAM vessel using (f). a–e were created with BioRender.com.
