Fig. 1: Schematic representation of the POPSIM concept.
From: Projective oblique plane structured illumination microscopy
A In OPSIM, a structured light sheet (numerical simulation of the intensity pattern shown below) emerges from a high numerical aperture lens. The structured light sheet can be azimuthally rotated, phase stepped, and scanned laterally to acquire stacks. B Schematic illustration of projection imaging. The light-sheet and focal plane are rapidly swept through the sample, covering the sample (light-blue parallelogram). If at least one sweep occurs during a camera exposure, a sum projection is formed (right). By synchronously shearing the images on the camera during the focal sweep, a projection along the z-axis can be formed (bottom). C In OPSIM stacks under different phases of the structured light-sheet are acquired for three azimuthal directions (top). In POPSIM, each volume is projected along the z-axis (bottom). D In OPSIM, volumes acquired under different directions are computationally registered in 3D to each other. In POPSIM, the corresponding projections are registered in 2D to each other.
