Fig. 2: Nanoparticle characterization.
From: Self-assembled peptide-dye nanostructures for in vivo tumor imaging and photodynamic toxicity
A MANTA measurements of the native iRGD peptide with methylene blue show that no nanoparticles were formed. These data show a characteristic MANTA image (i.e., darkfield microcopy image) as the background of the plot. B The iRGD-DD and methylene blue form 180 nm nanoparticles over 90 minutes. C The iRGD-WW and methylene blue form 150 nm nanoparticles over 90 min. Data in panels A–C are overlaid with scattering light detected by the MANTA, corroborating the formation of growing nanoparticles. D TEM micrographs show that the iRGD-DD + methylene blue and iRGD-WW + methylene blue samples formed into branched iRGD nanoparticles (iRGD-BNPs) and spherical iRGD nanospheres (iRGD-SNPs), respectively. The molecular dye, peptides, and combination between the dye and the native iRGD did not form assemblies with characteristic shapes.
