Fig. 2: Schematics of the MWIR apparatus. | npj Imaging

Fig. 2: Schematics of the MWIR apparatus.

From: Nondestructive, longitudinal, 3D oxygen imaging of cells in a multi-well plate using pulse electron paramagnetic resonance imaging

Fig. 2

a A rectangular resonator without air directors and covers. b a cross-section of multi-well plate enclosure and the resonator, air flow is shown using yellow direction arrows. c a resonator with air director (left) and cover (right) installed; a two-channel air intake is visible. d Three 8-well stripwells from a 96-well plate with columns C to F containing 150 μL of culture medium with 1 mM OX071. The 12 wells outlined in green were used for pO2 imaging. e Thermal image of the multi-well plate enclosure immediately after removal from MWIR showing that the temperature was maintained at 37 °C. f Relative humidity plot shows that a gas flow rate of 30 sccm sets the humidity at 100% while 3.75 sccm sets the humidity at 70%. g Representative pO2 maps (slice #4 at ~ 3.2 mm from the bottom of the well) of the middle 12 wells (columns C to F) filled with PBS during reoxygenation with 95% air + 5% CO2 after N2 bubbling. SCCM (standard cubic centimeter per min).

Back to article page