Fig. 3: Oxygen imaging of HEK-293 cells in MWIR. | npj Imaging

Fig. 3: Oxygen imaging of HEK-293 cells in MWIR.

From: Nondestructive, longitudinal, 3D oxygen imaging of cells in a multi-well plate using pulse electron paramagnetic resonance imaging

Fig. 3

a The pattern of cell seeding for control (CM), 5 K, and 50 K cells per well (n = 4). Each well had 150 μL of medium. b Representative pO2 maps of wells (slice #4, ~3.2 mm from the bottom of the well) in the transverse plane at t = 0 h (first imaging experiment). c Box plot of pO2 for each cell density; differences are statistically significant (p ≤ 0.001). d Cell viability measured by MTT assay after 24 h of imaging shows no significant difference between cells in the MWIR and BOD incubator at 5 K and 50 K densities (p > 0.05). e pH of the medium from wells that were in MWIR and in the incubator after 24 h. f Cell morphology after 24 h imaging from MWIR and BOD.

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