Fig. 5: The sensitivity of pO2 imaging. | npj Imaging

Fig. 5: The sensitivity of pO2 imaging.

From: Nondestructive, longitudinal, 3D oxygen imaging of cells in a multi-well plate using pulse electron paramagnetic resonance imaging

Fig. 5

a Six different cell densities of HEK-293 cells (CM (0 K), 10 K, 25 K, 50 K, 75 K, and 100 K cells per well) were seeded in the given pattern (n = 2). b A representative pO2 map at t = 0 h showing visual assessment of cell metabolic activity. The image is taken ~3.2 mm above the bottom of the well. c Box plot of pO2 as a function of cell density. The mean and standard error obtained from the pO2 map for CM (144.06 ± 4.82 torr), 10 K (116.61 ± 1.45 torr), 25 K (49.58 ± 0.74 torr), 50 K (27.97 ± 0.70 torr), 75 K (30.15 ± 0.94 torr), and 100 K (32.15 ± 1.18 torr), (n = ~ 200–300 voxels per well, n = 2 per cell density). There is a statistically significant difference (p < 0.0001) in pO2 between CM vs 10 K, 10 K vs 25 K, 25 K vs 50 K, 50 K vs 75 K, and 75 K vs 100 K. d The change in pO2 as a function of time. e MTT assay after 4 h revealed no significant differences between MWIR and BOD (p > 0.05), except for 100 K cells (p ≤ 0.05). f Morphological assessment of cells in MWIR and BOD after 4 h of measurements.

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