Fig. 6: Assessment of pO2 for HEK- 293 cells cultured 4 h in the BOD incubator. | npj Imaging

Fig. 6: Assessment of pO2 for HEK- 293 cells cultured 4 h in the BOD incubator.

From: Nondestructive, longitudinal, 3D oxygen imaging of cells in a multi-well plate using pulse electron paramagnetic resonance imaging

Fig. 6

a The layout of the wells for 0 K (CM), 10 K, 50 K, and 100 K cells per well (n = 3). Representative pO2 maps at 4 h of cells in (b) MWIR and (c) BOD incubator taken ~ 3.2 mm from the bottom of the wells. d pO2 quantification between the MWIR and BOD incubator. The mean and standard error of pO2 are: MWIR- CM (148.27 ± 2.97 torr), 10 K (91.77 ± 1.16 torr), 50 K (34.9 ± 0.95 torr), 100 K (35.98 ± 0.99 torr) or BOD -CM (106.42 ± 1.00 torr), 10 K (110.69 ± 1.85 torr), 50 K (37.10 ± 1.57 torr), 100 K (25.95 ± 1.05 torr), (n = 300–750 voxels per well, n = 3 per cell density). There is a significant difference (p < 0.0001) in pO2 between different cell densities. The MWIR vs BOD p-values among wells are as follows: CM, p = 0.1938; 10 K, p = 0.0179; 50 K, p = 0.5495; 100 K, p = 0.01863; MWIR: CM vs 10 K, p = 0.0015; 10 K vs 50 K, p = 0.00014; and 50 K vs 100 K p = 0.9415.

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