Fig. 7: Oxygen imaging of Jurkat cells using MWIR. | npj Imaging

Fig. 7: Oxygen imaging of Jurkat cells using MWIR.

From: Nondestructive, longitudinal, 3D oxygen imaging of cells in a multi-well plate using pulse electron paramagnetic resonance imaging

Fig. 7

a The cell seeding pattern of Jurkat cells CM, 5 K, and 50 K cells per well (n = 4). b A representative pO2 map taken at t = 0 h (~ 3.2 mm from the bottom of the well). c The box plot of pO2 values from t = 0 h. Differences in pO2 are statistically significant: CM vs 5 K, p = 0.0002 and 5 K vs 50 K, p < 0.0001. d The MTT assay after 4 h of imaging showed no significant difference in cell viability between the MWIR and BOD incubator (p > 0.05). e, f pH and cell morphology confirm that the MWIR emulates incubator-like conditions to enable in situ oxygen mapping of live cells.

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