Fig. 9: 3D oxygen imaging of HEK-293 cells, Jurkat cells and Jurkat cells in VitroGel. | npj Imaging

Fig. 9: 3D oxygen imaging of HEK-293 cells, Jurkat cells and Jurkat cells in VitroGel.

From: Nondestructive, longitudinal, 3D oxygen imaging of cells in a multi-well plate using pulse electron paramagnetic resonance imaging

Fig. 9

a The cell seeding pattern of 0 K (CM), 5 K, and 50 K cells per well (n = 4). b Three slices from the bottom to top showing the change in pO2 over depth for HEK-293 cells. c pO2 maps of three wells with 50 K cells denoted as A, B, and C in b for better visualization of vertical gradient. For each well, the central slice in the yz plane was cut and reported. The central slice was identified by choosing the slice with the highest number of pixels in the “y” direction. d The pO2 at the bottom, middle, and top of the wells at t = 0 for CM, 5 K and 50 K HEK-293 cells. e The pO2 for 50 K cells at the bottom, middle and top of the wells for HEK-293, Jurkat and Jurkat seeded in VitroGel at t = 0 and 4 h. There is a significant difference in pO2 between the bottom of the well and the top of the well for all cases except for Jurkat cells at 4 h.

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