Fig. 7: Uptake of FAPI in in healthy and inflamed murine paws. | npj Imaging

Fig. 7: Uptake of FAPI in in healthy and inflamed murine paws.

From: Increased imaging ligand hydrophilicity and improved pharmacokinetic properties provides enhanced in vivo targeting of fibroblast activation protein

Fig. 7

A Greater reductions in diseased paw mean fluorescent intensities (MFI) suggest FAPI-AF647 is a better blocker and stronger in vivo FAP engager than FAPI alone. B This trend is also observed with addition of the albumin-binding small molecule. Absolute MFI is also higher, likely due to increased perfusion-driven signal. Slower clearance rates also enable imaging out to longer times, but also lead to increased non-specific signal in healthy paws compared to that observed with FAPI. C After background subtraction, FAPI-AF647 was demonstrated to block > 2-fold more target specific signal when compared to FAPI for non-albumin (left) and albumin binding (right) versions of FAPI-800CW at 24 h (Mean +/- SEM). ns: non-significant.

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