Table 1 Physicochemical parameters for FAPI conjugates used during in vivo applications, including MW, cLogP and plasma protein binding

From: Increased imaging ligand hydrophilicity and improved pharmacokinetic properties provides enhanced in vivo targeting of fibroblast activation protein

Reagents

MW (Da)

cLogP

% Protein Bound ± SD

FAPI

486.51

0.3

44.7 ± 3.1

FAPI-ALB

1177.2

1.0

98.9 ± 0.2

FAPI-AF647

1328.52

−0.3

20 ± 12.7

FAPI-ALB-AF647

2019.21

−1.0

99.7 ± 0.1

  1. Addition of sulfonated dyes alone increases hydrophilicity. Addition of the albumin-binding small molecule significantly increases plasma protein binding.
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