Table 2 A Cell surface determined binding affinities (KD) for directly measured and indirectly* calculated FAP conjugates. B Flow cytometry using saturating concentrations of FAPI-AF488 and MESF beads to quantify surface expression in overexpressing and endogenous cells

From: Increased imaging ligand hydrophilicity and improved pharmacokinetic properties provides enhanced in vivo targeting of fibroblast activation protein

Reagents

KD (nM) ± SEM

A

FAPI

6*

FAPI-AF488

4.8 ± 0.5

FAPI-AF647

5.0 ± 0.9

FAPI-800CW

8*

FAPI-ALB

3.6*

FAPI-ALB-AF647

3.4 ± 0.4

FAPI-ALB-800CW

9.9*

B

Cell line

Receptors/cell

RAFLS

~25,000

HEK293-hFAP

~1,000,000

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