Fig. 7: Combination of MALDI-MSI with 2PLSM identifies differential composition of proteomic clusters in chaotic and organised regions of LSCC compared to RSCC. | npj Imaging

Fig. 7: Combination of MALDI-MSI with 2PLSM identifies differential composition of proteomic clusters in chaotic and organised regions of LSCC compared to RSCC.

From: MALDI imaging combined with two-photon microscopy reveals local differences in the heterogeneity of colorectal cancer

Fig. 7

A Segmentation by using k-means results in differential proteomic clusters in the organised regions of LSCC compared to RSCC. The spectra and distance of peptide clusters are shown beside the segmentation maps. B Segmentation by using k-means results in differential proteomic clusters in the chaotic regions of LSCC compared to RSCC. The spectra and distance of peptide clusters are shown beside the segmentation maps. C Quantification of proteomic clusters in the organised regions highlights the differences in percentage areas occupied by each cluster between LSCC and RSCC. D Quantification of proteomic clusters in the chaotic regions highlights the differences in percentage areas occupied by each cluster between LSCC and RSCC. E The percentage area occupied by the organised and chaotic regions in the LSCC compared to RSCC is shown. N = 14, *signifies p < 0.05, **signifies p < 0.01.

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