Fig. 3: Applications of light sheet microscopy for studies of structures and dynamics in biology.
a Time-lapse imaging of multicellular systems with open-top dual-view light-sheet microscope54. Top row: Intestinal organoids expressing the Fucci2-reporter (hGem-mVenus and hCdt1-mCherry). Bottom row: liver organoids expressing mg-GFP and H2B-mCherry. Scale bars: 50 µm. Figure adapted from Moos, F. et al. Nat. Methods 21, 798–803 (2024). Reprinted with permission from Springer, under Creative Commons Attribution 4.0 International License. b 3D imaging of embryogenesis dynamics with lattice light sheet (LLS) microscopy73. Top-left: DE-cadherin during dorsal closure in Drosophila development. Top-right: Myosin II during dorsal closure. Bottom row: sGMCA during dorsal closure. Bounding boxes are 86 × 80 × 31 , 80 × 80 × 26 , and 73 × 73 × 26 µm, respectively. From Chen, B.-C. et al. Science 346, 1257998 (2014). Reprinted with permission from AAAS. c Live-cell 3D super-resolution (SR) imaging with LLS microscopy combined with structured illumination10. Left: live-cell 3D SR imaging of mitochondria (Skylan-NS-TOM20) in a COS-7 cell. Right: time-lapse distribution of Golgi-resident enzyme Skylan-NS-Mann II at three exemplary time points in a U-2 OS cell. Scale bars: 5 µm and 3 µm, respectively. From Li, D. et al. Science 349, aab3500 (2015). Reprinted with permission from AAAS. d SR imaging of nucleosomes with HIST microscopy85. Individual nucleosomes are assigned chromatin density classification according to their localizations for study of nucleosome organizations. Scale bar: 1 µm. Figure adapted from Daugird, T. A. et al. Nat. Commun. 15, 4178 (2024). Reprinted with permission from Springer, under Creative Commons Attribution 4.0 International License. e 3D high-resolution large-volume imaging with DaXi96. Top row: a zebrafish larva. Bottom row: Drosophila fly egg chambers. Scale bars: 100 µm. Figure adapted from Yang, B. et al. Nat. Methods 19, 461–469 (2022). Reprinted with permission from Springer, under Creative Commons Attribution 4.0 International License. f 3D high-density SR imaging of cellular structures with soTILT3D107. Left: 3D reconstruction of lamin A/C and mitochondria (TOMM20) in a U-2 OS cell. Right: 3D multi-target SR reconstruction of an entire cell nucleus labeled for lamin B1, LAP2, and lamin A/C. Scale bars: 10 µm.
