Fig. 3: Near infrared fluorescence lymphangiography measurement in mice across age, sex, and lymphatic position.

A An example image from a series of pictures comprising the 5-min lymphangiography recording showing 8 regions of interest tiled across the lymphatic vessel. The peak-and-valley plot and spectrogram are shown in the baseline position, afterload position (head up), and offload position (head down). B The model estimate +/− SEM from linear mixed modeling for peak and valley frequency (min⁻¹), wavelet frequency (min⁻¹), peak and valley amplitude (a.u.), wavelet amplitude (a.u.) and peak and valley mean intensity (a.u.) are given for the baseline, afterload and offload positions in adult (blue line) and old (red line) mice (N = 8 adult mice, N = 8 old mice), data collapsed across sex. Tukey’s HSD was used to determine statistical differences between group means for posthoc pairwise comparisons. C The same data as in (B) is shown split by sex, with green solid line adult female (N = 5), green dotted line old female (N = 4), purple solid line adult male (N = 3), purple dotted line old male (N = 4). Tukey’s HSD was used to determine statistical differences between group means for posthoc pairwise comparisons.