Fig. 4: Intravital epifluorescence microscopy. | npj Imaging

Fig. 4: Intravital epifluorescence microscopy.

From: A multiresolution approach with method-informed statistical analysis for quantifying lymphatic pumping dynamics

Fig. 4: Intravital epifluorescence microscopy.The alternative text for this image may have been generated using AI.

A A lymphatic vessel afferent to the popliteal lymph node in a live mouse is filled with fluorescein isothiocyanate dye by interdermal injection of the mouse foot and imaged on an epifluorescence microscope. A single region of interest is captured and diameter changes over time are recorded. An example lymphatic vessel in diastole and systole is shown. B Example data in a young mouse. The average vessel diameter over time is measured over 300 s. The spectrogram below shows the instantaneous frequency throughout the recording in an adult mouse. Below, example of diameter vs. time and instantaneous frequency vs. time for an old mouse. C Mean +/− SEM of contraction frequency (peak-and-valley analysis), frequency (peak-and-valley analysis), amplitude (peak-and-valley and wavelet analysis), and average diameter (peak-and-valley analysis) for young mice (blue) (n = 9) and old mice (red) (n = 8). Unpaired t-test was used to determine statistical differences between group means for posthoc pairwise comparisons.

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