Fig. 3: SIM reconstruction of evolving mitochondrial cristae at 569 nm emission at different SNR.
From: Approaching maximum resolution in structured illumination microscopy via accurate noise modeling
a 1024 × 454 pseudo-widefield (Wf) image of the mitochondria network in HeLa cells labeled with PKmito RED dye. Scale bar 5 μm. b SIM raw image (R) and reconstructions using Wiener filter (Wi), FISTA-SIM (Fi), and B-SIM (B) methods. γ parameter shows the average photon count in the image. Scale bar 2 μm. Line cuts from the three reconstructions are compared in the rightmost panel. c Same as in b at intermediate SNR. In the raw image, the left triangular half shows an image with the same color scaling as in (b). The right half rescales the image for visual convenience. We do not show line cuts for the FISTA-SIM reconstruction as it has significantly deteriorated. d Same as in c but at very low SNR. We do not compare line cuts as both FISTA- and Wiener-SIM reconstructions have significant noise amplification. The three sets of raw images at different SNR were taken at approximately 1.2 s intervals.
