Fig. 1: Study design.

The study comprised n = 16 domestic ferrets, divided into three experimental groups: (a) non-exposed (non-exp, beige), ferrets that underwent Fe-PyC3A magnetic resonance imaging (MRI) and were euthanized for ex vivo readouts without viral exposure; (b) 3 dpe (blue), ferrets that underwent Fe-PyC3A MRI before Ebola virus (EBOV) exposure and at 3 dpe and were then euthanized for ex vivo readouts; (c) dt (pink), ferrets that underwent Fe-PyC3A MRI before EBOV exposure (pre-exp), at 3 dpe and at dt and were then euthanized for ex vivo readouts. 3 dpe, 3 d post-exposure; dt, day terminal, pre-exp, pre-exposure; RT-qPCR, real-time reverse transcription polymerase chain reaction.