Fig. 4: Experimental design and MPI signal quantification for fiducials and liver/spleen in a representative mouse.
From: Spillover can limit accurate signal quantification in MPI
a (i) Design of a 3D-printed MPI bed for precision-aligned tracking of fiducials during repeated in vivo imaging. (ii) A mouse positioned on a customized MPI bed for repeated in vivo MPI sessions, with two distinct fiducials with precise alignment. The tail vein was catheterized for repeated injection and secured in place throughout the procedure. The syringe was filled with 400 µl of ferucarbotran at 200 µg Fe/ml. (iii) Representative MPI after the first injection. b MPI signal quantification of fiducials and the liver/spleen at 40-min intervals following sequential injections, free from any other hotspot interference. The first two injections contained 20 µg of iron, while the third dose doubled to 40 µg, totaling 80 µg of iron.
